After administering a drug in rat, pharmacokinetic study may be required without sacrificing the animal. In that case blood may be collected from tail vein.
Iodine tincture, alcohol 75%, sterilized operating knife, micropipette with microtips, microcentrifuge and microcentrifuge tubes (0.5ml capacity).
- Drug solution and in situ gel (equvalent to 15mg drug) is injected i.m. into the right musculus rectus of the rat.
- After administering the drug to the rats (intra muscularly, per oral or intra peritoneally) briefly, the tested animal is put in a body restrainer. The tail of the rat is warmed by an incandescent lamp in order to make the blood fill the tail vein.
- The tail is cleaned by iodine tincture and 75% alcohol before it is cut by a sterilized operating knife. The cut is near the end of the rat tail. In order to make blood flow out of the tail vein, a moderate pressure on the tail is applied by the thumb of the operator.
- Subsequently, 0.5 ml blood is collected at each interval. The blood samples are immediately centrifuged at 5000 rpm for 10 min. The separated plasma sample is collected and frozen at - 400C before being analyzed by HPLC. The plasma samples are collected at 0.25, 0.5, 0.75, 1, 3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 days, respectively.
- Xin, C., Lihong, W., Qiuyuan, L., Hongzhuo, L., 2014. Injectable long-term control-released in situ gels of hydrochloric thiothixene for the treatment of schizophrenia: Preparation, in vitro and in vivo evaluation. International Journal of Pharmaceutics 469, 23–30.