Liposomes Introduction and Preparation by Zoraiz Haider
Lipids for Liposomes: Selection, Preparation and Application
Di Bush, Avanti Polar Lipids
Microfluidic Production of Liposomes
Yvonne Perrie, Strathclyde University
- Microfluidic production of liposome
- Visual appearance of liposome under microscope, particile size distribution,
- Phase transition temperature
Plasma membrane | Transition temperature
By Shantanu Kolhe
- Behavior of lipids in the fluidity of plasma membrane.
Cell membrane fluidity and role of cholesterol in membrane fluidity
By Shomu's Biology
- Cholesterol has a big bulky hydrophobic body and a very small hydrophilic group (-OH),
- In this video the role of Cholesterol in maintaining the membrane fluidity is discussed.
- At higher temperature, cholesterol decreases membrane-fluidity. Cholesterol provides large surface area for hydrophobic interaction with the long tails of the surrounding lipid chains of the phospholipids. Thus makes the lipid membrane more rigid.
- At lower temperature, cholesterol molecules push the closely packed long tails of the phospholipids. Thus cholesterol increases the membrane-fluidity.
- Therefore, Cholesterol molecules provide the optimum fluidity of the lipid bilayers at both higher and lower temperatures.
Lab 5 Synthesis and Characterization of Liposomes
Making Liposomes
HOW TO MAKE LIPID NANOPARTICLES - A day in the life of a PhD
Making Liposomal Vitamin C: a tutorial
by Henrik Eiriksson
Drying temperature
The temperature of the water bath during the drying of the lipid phase in a rotary evaporator typically depends on the solvent used to dissolve the lipids. For common solvents like chloroform or methanol, the water bath temperature is usually set slightly below the boiling point of the solvent to ensure efficient evaporation without overheating. For example:
- Chloroform: Around 40–50°C.
- Methanol: Around 50–60°C.
Maintaining the right temperature is crucial to prevent degradation of the lipids and ensure proper solvent removal.
Glass Transition Temperature
The glass transition temperature (Tg) plays a crucial role in the preparation and stability of liposomes, especially during processes like lyophilization (freeze-drying). Tg represents the temperature at which a material transitions from a glassy state to a rubbery state. Here's why it's important:
- Stability During Freeze-Drying: Liposomes are often freeze-dried to improve their shelf life. During this process, Tg helps determine the temperature at which the liposomal formulation remains stable without collapsing or losing its structural integrity.
- Cryoprotectants and Lyoprotectants: Tg guides the selection of excipients like cryoprotectants and lyoprotectants, which protect liposomes during freezing and drying phases.
- Encapsulation Efficiency: Tg influences the encapsulation of drugs within liposomes, ensuring that the active pharmaceutical ingredients remain stable and effective.
- Storage Conditions: Knowing the Tg of a liposomal formulation helps in setting appropriate storage conditions to maintain its stability and prevent degradation.
