Serial dilution of bacterial culture MIC

 Serial dilution of bacterial culture

LINK

Broth Microdilution assay - How to determine the MIC (Minimum Inhibitory Concentration)

Henrik's Lab

LINK

MIC (Broth Microdilution) Testing

By TheRubinLab

LINK

McFarland Standard

In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing.

McFarland nephelometer standards:

McFarland Standard No.	0.5	1	2	3	4
1.0% barium chloride (ml)	0.05	0.1	0.2	0.3	0.4
1.0% sulfuric acid (ml)	9.95	9.9	9.8	9.7	9.6
Approx. cell density (1X10^8 CFU/mL)	1.5	3.0	6.0	9.0	12.0
% transmittance*	74.3	55.6	35.6	26.4	21.5
Absorbance at 600nm*	0.08 to 0.1	0.257	0.451	0.582	0.669

• Preparation of 1.0% Barium chloride suspension: 
0.05 mL of 1.175% barium chloride dihydrate (BaCl2•2H2O)

How to perform serial dilutions and make microbial suspensions using McFarland Standards

LINK